A magnetic relaxation switching and visual dual-mode sensor for selective detection of Hg²⁺ based on aptamers modified Au@Fe₃O₄ nanoparticles

The solvated mercuric ion (Hg²⁺) from industrial pollutants are highly toxic to the ecological environment and human health. Driven by urgent need for the selective and sensitive detection of Hg²⁺, a magnetic relaxation switching (MRS) based on Fe₃O₄ nanoparticles (NPs) was designed. Practically, the concentrations of Hg²⁺ in industrial pollutant is usually much higher than the detection range. Thus, gold nanoparticles (AuNPs) were synthesized on the surface of Fe₃O₄ NPs to enable the visual detection of Au@Fe₃O₄ NPs. The presence of Hg²⁺ in sample can specifically cause the aggregation of Au@Fe₃O₄-aptamers NPs through T-Hg²⁺-T base pairs, leading to the change in transverse relaxation time T₂ value of detection solution. The MRS sensor showed excellent response for Hg²⁺ ions in the range of 10 nM-100 nM and 100 nM to 5 μM. A highly sensitive and selective measurement of Hg²⁺ was obtained with a limit of detection of 2.7 nM. Noticeably, the visual detection can qualitatively analyze the Hg²⁺ beyond 5 μM by naked eye without advanced instrumentation and skilled operators.