Detection of Small Subsets of CD4+ Lymphocytes with SmartFlare Nanoprobes

A P Toptygina; R Sh Zakirov; K S Kapitanova; E L Semikina

doi:10.1007/s10517-019-04689-5

Detection of Small Subsets of CD4⁺ Lymphocytes with SmartFlare Nanoprobes

Bull Exp Biol Med. 2019 Dec;168(2):270-274. doi: 10.1007/s10517-019-04689-5. Epub 2019 Nov 29.

Authors

A P Toptygina¹, R Sh Zakirov², K S Kapitanova³, E L Semikina²

Affiliations

¹ G. N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being, Moscow, Russia. [email protected].
² National Medical Research Center for Children's Health, Ministry of Health of the Russian Federation, Moscow, Russia.
³ G. N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being, Moscow, Russia.

PMID: 31784846
DOI: 10.1007/s10517-019-04689-5

Abstract

SmartFlare technology allows detection of mRNA in single living cells. We studied the possibility of using SmartFlare nanoprobes for detection of small subsets of CD4⁺ lymphocytes. It was found that SmartFlare allows detection of transcriptional master regulators of major CD4⁺T helper subsets in living human lymphocytes. Nanoprobes labeled with Cy5 fluorophore were better detected by flow cytometry than nanoprobes labeled with Cy3. Appropriate time of lymphocyte incubation with SmartFlare probes was 24 h.

Keywords: SmartFlare; subsets of CD4+ lymphocytes; transcriptional factors.

MeSH terms

Carbocyanines / chemistry
Flow Cytometry / methods*
Fluorescent Dyes / chemistry*
Gold / chemistry
Humans
Metal Nanoparticles / chemistry
RNA, Complementary / genetics*
RNA, Messenger / genetics*
T-Lymphocyte Subsets / cytology*
T-Lymphocytes, Helper-Inducer / cytology*

Substances

Carbocyanines
Fluorescent Dyes
RNA, Complementary
RNA, Messenger
cyanine dye 3
cyanine dye 5
Gold