(Pyridoxylated hemoglobin)-(polyoxyethylene) conjugate solution as blood substitute for normothermic whole body rinse-out

Biomater Artif Cells Artif Organs. 1988;16(1-3):261-70. doi: 10.3109/10731198809132575.

Abstract

In order to investigate a new possibility for artificial blood with oxygen-carrying capability to be applied to other than mere supplementation, normothermic whole body rinse-out in which artificial blood deriving from perfluorochemical emulsion, Fluosol-DA 20% (Green Cross Co., Ltd., Osaka, Japan) or stabilized hemoglobin solution, (pyridoxylated hemoglobin)-(polyoxyethylene) conjugate solution (Ajinomoto Co., Ltd., Tokyo, Japan) were used as rinsing fluid for a blood purification experiment. Replacement either with approximately 150 ml/kg of Fluosol-DA or stabilized hemoglobin solution showed effective removal of digoxin at a reduction rate of 96.3% or 92.2%, respectively. However, when Fluosol-DA was used, a certain amount of perfluorochemical should be retrieved by centrifugation to avoid a possible toxic effect on the reticulo-endothelial system. Even though 3 out of 6, and 3 out of 8 dogs, respectively, survived for a long period after the procedure, the experimental dogs were very susceptible to infection.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Coagulation Disorders / chemically induced
  • Blood Substitutes / therapeutic use*
  • Blood Substitutes / toxicity
  • Chemical and Drug Induced Liver Injury
  • Digoxin / blood
  • Digoxin / toxicity
  • Dogs
  • Drug Combinations / therapeutic use
  • Drug Combinations / toxicity
  • Exchange Transfusion, Whole Blood*
  • Fluorocarbons / therapeutic use
  • Fluorocarbons / toxicity
  • Hematocrit
  • Hemoglobins* / metabolism
  • Hydroxyethyl Starch Derivatives
  • Kidney Diseases / chemically induced
  • Polyethylene Glycols* / metabolism
  • Solutions

Substances

  • Blood Substitutes
  • Drug Combinations
  • Fluorocarbons
  • Hemoglobins
  • Hydroxyethyl Starch Derivatives
  • Solutions
  • pyridoxalated-hemoglobin-polyethylene glycol conjugate
  • Polyethylene Glycols
  • Digoxin
  • glucose, glycerol, hydroxyethyl starch, perfluorodecalin, perfluorotripropylamine, pluronic F-68, salts, yolk phospholipids drug combination