Germline-Transmitted Genome Editing Methodology in Arabidopsis thaliana Using TAL Effector Nucleases

Joachim Forner

doi:10.1007/978-1-0716-0183-9_3

Germline-Transmitted Genome Editing Methodology in Arabidopsis thaliana Using TAL Effector Nucleases

Methods Mol Biol. 2020:2094:23-30. doi: 10.1007/978-1-0716-0183-9_3.

Author

Joachim Forner¹

Affiliation

¹ Organelle Biology, Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam, Germany. [email protected].

PMID: 31797287
DOI: 10.1007/978-1-0716-0183-9_3

Abstract

TAL effector nucleases (TALENs) are powerful tools to create specific knockout mutants in plants. The use of an optimized TALEN backbone and the choice of promoters that are strongly active in the stem cells of the shoot apical meristem are key to a high rate of heritable targeted mutations. Recommendations for construct design and screening for mutants are given in this chapter.

Keywords: Arabidopsis thaliana; Genome editing; Germline; Heritable; Knockout; NHEJ; Stem cell-specific promoter; TALEN.

MeSH terms

Arabidopsis / genetics*
Gene Editing / methods*
Germ Cells / metabolism*
Mutagenesis, Site-Directed / methods*
Plants, Genetically Modified / genetics*
Promoter Regions, Genetic
Transcription Activator-Like Effector Nucleases / genetics
Transcription Activator-Like Effector Nucleases / metabolism*

Substances

Transcription Activator-Like Effector Nucleases