Mu opioid receptors inhibit GABA release from parvalbumin interneuron terminals onto CA1 pyramidal cells

Caifeng Shao; Pei Chen; Qian Chen; Mingwei Zhao; Wei-Ning Zhang; Kun Yang

doi:10.1016/j.bbrc.2019.12.013

Mu opioid receptors inhibit GABA release from parvalbumin interneuron terminals onto CA1 pyramidal cells

Biochem Biophys Res Commun. 2020 Feb 19;522(4):1059-1062. doi: 10.1016/j.bbrc.2019.12.013. Epub 2019 Dec 6.

Authors

Caifeng Shao¹, Pei Chen², Qian Chen³, Mingwei Zhao³, Wei-Ning Zhang⁴, Kun Yang⁵

Affiliations

¹ Department of Anesthesiology, Nantong Hospital of Traditional Chinese Medicine, Nantong, Jiangsu, 226001, China; Department of Anatomy, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
² Department of Anatomy, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, China; Class 2017, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
³ Department of Anatomy, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
⁴ Department of Basic Medical Sciences, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
⁵ Department of Anatomy, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, China; Department of Neurology, University of Maryland School of Medicine, Baltimore, MD, 21201, USA. Electronic address: [email protected].

PMID: 31818459
DOI: 10.1016/j.bbrc.2019.12.013

Abstract

Selectively activating (by optogenetics) parvalbumin-expressing (PV) interneurons induces GABA release onto CA1 pyramidal cells. Here we report that this release was attenuated by presynaptic mu opioid receptors (MORs) activation. On the other hand, conventional electric shock, presumably activating non-selectively presynaptic GABAergic terminals, also induced GABA release; however, this release showed relatively limited depression by MORs activation. The data suggest that MORs specifically inhibit GABA release from PV terminals and therefore, further support the idea that MORs contribute to homeostasis in CA1 neuro-circuit.

Keywords: GABA; Hippocampus; Mu opioid receptors; Optogenetics; Parvalbumin; Whole-cell recordings.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CA1 Region, Hippocampal / cytology*
Enkephalin, Ala(2)-MePhe(4)-Gly(5)- / pharmacology
Female
Interneurons / drug effects
Interneurons / metabolism*
Male
Mice, Transgenic
Parvalbumins / metabolism*
Pyramidal Cells / drug effects
Pyramidal Cells / metabolism*
Receptors, Opioid, mu / metabolism*
gamma-Aminobutyric Acid / metabolism*

Substances

Parvalbumins
Receptors, Opioid, mu
Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
gamma-Aminobutyric Acid