Echovirus types 4 and 33 and coxsackievirus type B1 were recovered from vesicular lesions in four adults. Patient 1 had cutaneous localized vesicles, patient 2 had a recurrent cutaneous vesicle, and patients 3 and 4 had mucosal vesicles. Three of the patients were suspected of having herpesvirus lesions. One of the patients was a human immunodeficiency virus type 1-seropositive man, and the enterovirus infection was the first clinical manifestation. Our results underline the importance of virological diagnosis before treatment with acyclovir, especially for immunocompromised patients.