The alternative cap-binding complex is required for antiviral defense in vivo

PLoS Pathog. 2019 Dec 19;15(12):e1008155. doi: 10.1371/journal.ppat.1008155. eCollection 2019 Dec.

Abstract

Cellular response to environmental challenges requires immediate and precise regulation of transcriptional programs. During viral infections, this includes the expression of antiviral genes that are essential to combat the pathogen. Transcribed mRNAs are bound and escorted to the cytoplasm by the cap-binding complex (CBC). We recently identified a protein complex consisting of NCBP1 and NCBP3 that, under physiological conditions, has redundant function to the canonical CBC, consisting of NCBP1 and NCBP2. Here, we provide evidence that NCBP3 is essential to mount a precise and appropriate antiviral response. Ncbp3-deficient cells allow higher virus growth and elicit a reduced antiviral response, a defect happening on post-transcriptional level. Ncbp3-deficient mice suffered from severe lung pathology and increased morbidity after influenza A virus challenge. While NCBP3 appeared to be particularly important during viral infections, it may be more broadly involved to ensure proper protein expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Influenza A virus / immunology
  • Mice
  • Mice, Knockout
  • Orthomyxoviridae Infections / immunology*
  • Orthomyxoviridae Infections / metabolism
  • Protein Biosynthesis / physiology
  • RNA Cap-Binding Proteins / immunology*
  • RNA Cap-Binding Proteins / metabolism*

Substances

  • RNA Cap-Binding Proteins

Grants and funding

This work was supported by the Max-Planck Free Floater program to A.P., the German research foundation to A.P. (PI1084/3 and TRR179 TP11) and P.S. (SFB 1160, project 13), the Lunbeck Foundation to L.R. (R198 2015 171), the Centre National de la Recherche Scientifique (CNRS), University and European Regional Development Fund (FEDER (N° 2016-00110366 and EX005756) to B.R., an ERC consolidator grant to A.P (ERC CoG ProDAP, 817798) and the Infect-Era and the German Federal Ministry of Education and Research (ERASE) to A.P. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.