Characterization of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1-7-4 viruses) in northern China

Changzhan Xie; Zhuo Ha; Fulong Nan; Ying Zhang; Han Zhang; Jinfeng Li; Ping Zhang; Jicheng Han; He Zhang; Xinyu Zhuang; Jinyong Zhang; Huijun Lu; Ningyi Jin

doi:10.1016/j.micpath.2019.103941

Characterization of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1-7-4 viruses) in northern China

Microb Pathog. 2020 Mar:140:103941. doi: 10.1016/j.micpath.2019.103941. Epub 2019 Dec 17.

Authors

Changzhan Xie¹, Zhuo Ha², Fulong Nan³, Ying Zhang³, Han Zhang⁴, Jinfeng Li³, Ping Zhang⁵, Jicheng Han⁶, He Zhang⁴, Xinyu Zhuang⁴, Jinyong Zhang¹, Huijun Lu⁷, Ningyi Jin⁸

Affiliations

¹ College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China; Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, 130117, China.
² Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, 130117, China; College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
³ College of Veterinary Medicine, Jilin University, Changchun, 130012, China.
⁴ Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, 130117, China.
⁵ Institute of Specialty, Chinese Academy of Agricultural Sciences, Changchun, 130117, China.
⁶ Medical College, Yanbian University, Yanji, 133002, China.
⁷ Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, 130117, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, Yangzhou, 225002, China. Electronic address: [email protected].
⁸ College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China; Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, 130117, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, Yangzhou, 225002, China. Electronic address: [email protected].

PMID: 31862391
DOI: 10.1016/j.micpath.2019.103941

Abstract

The porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of porcine reproductive and respiratory syndrome (PRRS). Disease outbreaks caused by NADC30-like PRRSV strains were a bit prevalent in China in recent years. In the present study, two newly emerged PRRSV strains, which were designated as PRRSV-ZDXYL-China-2018-1 and PRRSV-ZDXYL-China-2018-2 strains were found from piglets' lung tissues in Northern China. The virus belongs to lineage 1 of the PRRSV genotype 2 and is closely related to US strains that possess the open reading frame (ORF5) restriction fragment length polymorphism (RFLP) 1-7-4. The two strains were identified from infected weaning piglet herds in Zhaodong City, Heilongjiang province of China. The complete genome of the PRRSV-ZDXYL-China-2018-1 and PRRSV-ZDXYL-China-2018-2 strains were 15093 nt and 15110 nt, and shared 96.7%-97.0% and 97.1%-97.4% similarities with the US identified, ISU10 and NADC34 strains respectively. Then the PRRSV-ZDXYL-China-2018-1 strain was successfully isolated from the clinical sample. Our results demonstrate, that the emergence of ORF5 RFLP 1-7-4-like PRRSVs in China, could pose a significant challenge to PRRSV epidemic prevention.

Keywords: Genetic variation; PRRSV; Virulence; Whole genome sequencing.

MeSH terms

Animals
China / epidemiology
Disease Outbreaks / veterinary
Genetic Variation
Genome, Viral
Lung / virology
Phylogeny
Porcine Reproductive and Respiratory Syndrome / virology*
Porcine respiratory and reproductive syndrome virus* / genetics
Porcine respiratory and reproductive syndrome virus* / isolation & purification
Swine
Whole Genome Sequencing