Teneligliptin prevents doxorubicin-induced inflammation and apoptosis in H9c2 cells

Arch Biochem Biophys. 2020 Apr 15:683:108238. doi: 10.1016/j.abb.2019.108238. Epub 2019 Dec 24.

Abstract

Doxorubicin is a common chemotherapy treatment with numerous negative ramifications of use such as nephropathy and radiation-induced cardiotoxicity. Doxorubicin has been shown to cause overexpression of proinflammatory cytokines including MCP-1 and IL-1β via activation of the NF-κB pathway. Furthermore, apoptosis marked by dysregulation of the Bax/Bcl-2 ratio and oxidative stress and the production of reactive oxygen species (ROS) are also exacerbated by doxorubicin administration. Teneligliptin is part of the wider dipeptidyl peptidase-4 (DPP-4) inhibitor family which has until recently been almost exclusively used to treat type 2 diabetes mellitus. DPP-4 inhibitors such as teneligliptin control the overexpression of glucagon-like peptidase 1 (GLP-1) which has the downstream effects of general insulin resistance and high blood sugar levels. Our findings indicate a significant protective effect of teneligliptin against the aftereffects of doxorubicin as a chemotherapy treatment. This protective effect includes but is not limited to the reduction of inflammation and the mitigation of dysregulated apoptosis, as evidenced by reduced expression of IL-1β and MCP-1, inhibition of NF-κB activation, and improvement of the Bax/Bcl-2 ratio. The aim of the present study was to establish teneligliptin as a potentially useful agent for the treatment of radiation-induced cardiotoxicity, and our findings support this notion.

Keywords: Apoptosis; Cardiomyocytes; Doxorubicin; NF-κB; Oxidative stress; Teneligliptin.

MeSH terms

  • Animals
  • Antineoplastic Agents / adverse effects
  • Apoptosis / drug effects*
  • Blood Glucose / analysis
  • Cell Line
  • Cell Survival / drug effects
  • Chemokine CCL2 / metabolism
  • Dipeptidyl Peptidase 4 / metabolism
  • Doxorubicin / adverse effects*
  • Glucagon-Like Peptide 1 / metabolism
  • Humans
  • Inflammation*
  • Insulin Resistance
  • Interleukin-1beta / metabolism
  • Myocytes, Cardiac / drug effects*
  • Oxidative Stress
  • Pyrazoles / pharmacology*
  • Rats
  • Reactive Oxygen Species / metabolism
  • Receptors, KIR3DL1 / metabolism
  • Thiazolidines / pharmacology*

Substances

  • 3-(4-(4-(3-methyl-1-phenyl-1H-pyrazol-5-yl)piperazin-1-yl)pyrrolidin-2-ylcarbonyl)thiazolidine
  • Antineoplastic Agents
  • Blood Glucose
  • CCL2 protein, human
  • Chemokine CCL2
  • Interleukin-1beta
  • Pyrazoles
  • Reactive Oxygen Species
  • Receptors, KIR3DL1
  • Thiazolidines
  • Doxorubicin
  • Glucagon-Like Peptide 1
  • DPP4 protein, human
  • Dipeptidyl Peptidase 4