Apple polysaccharide protects ICR mice against colitis associated colorectal cancer through the regulation of microbial dysbiosis

Yuhua Li; Sheng Wang; Yang Sun; Wenqi Xu; Hongnan Zheng; Yan Wang; Yuan Tang; Xiaowei Gao; Can Song; Yin Long; Jiayun Liu; Li Liu; Qibing Mei

doi:10.1016/j.carbpol.2019.115726

Apple polysaccharide protects ICR mice against colitis associated colorectal cancer through the regulation of microbial dysbiosis

Carbohydr Polym. 2020 Feb 15:230:115726. doi: 10.1016/j.carbpol.2019.115726. Epub 2019 Dec 9.

Authors

Yuhua Li¹, Sheng Wang², Yang Sun³, Wenqi Xu², Hongnan Zheng⁴, Yan Wang², Yuan Tang⁴, Xiaowei Gao⁵, Can Song⁵, Yin Long⁶, Jiayun Liu⁶, Li Liu⁷, Qibing Mei⁸

Affiliations

¹ Department of Microbial and Biochemical Pharmacy, School of Pharmacy, Southwest Medial University, Luzhou, 646000 Sichuan, PR China; Department of Pharmacy, the First Naval Force Hospital of Southern Theatre Command, Zhanjiang, 524005 Guangdong, PR China.
² State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai, 200437, PR China; Shanghai Professional and Technical Service Center for Biological Material Drug-ability Evaluation, Shanghai, 200437, PR China.
³ Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, Department of Pharmacology, School of Pharmacy, the Fourth Military Medical University, Xi'an, 710032 Shaanxi, PR China.
⁴ Department of Pharmacy, the First Naval Force Hospital of Southern Theatre Command, Zhanjiang, 524005 Guangdong, PR China.
⁵ Department of Microbial and Biochemical Pharmacy, School of Pharmacy, Southwest Medial University, Luzhou, 646000 Sichuan, PR China.
⁶ Xijing Hospital, the Fourth Military Medical University, Xi'an, 710032 Shaanxi, PR China.
⁷ State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai, 200437, PR China; Shanghai Professional and Technical Service Center for Biological Material Drug-ability Evaluation, Shanghai, 200437, PR China. Electronic address: [email protected].
⁸ Department of Microbial and Biochemical Pharmacy, School of Pharmacy, Southwest Medial University, Luzhou, 646000 Sichuan, PR China. Electronic address: [email protected].

PMID: 31887919
DOI: 10.1016/j.carbpol.2019.115726

Abstract

The study tried to investigate whether apple polysaccharide (AP) could prevent colitis associated colorectal cancer (CACC) through the regulation of intestinal microbiota disorders. 10 % AP (w/v) was administrated to ICR mice by gavage for 15 wk. It was found that AP treatment protected against CACC in mice effectively. The level of Lactobacillus in the intestine of AOM/DSS-treated mice was significantly decreased and that of Fusobacterium increased; while AP could reverse this trend and increase the intestinal microbiota diversity. The number of T cells and macrophages in the colon tissue of mice in AOM/DSS group elevated; while AP could reduce the number of these cells significantly. AP suppressed nuclear aggregation of β-catenin, inhibited the activation of Wnt pathway in colon tissues. These data suggest that AP prevented ICR mice from CACC at least in part through regulating intestinal flora disorder and Wnt pathway.

Keywords: Apple polysaccharide; Bacteria; Colitis associated colorectal cancer.

MeSH terms

Animals
Colitis* / diet therapy
Colitis* / microbiology
Colonic Neoplasms* / diet therapy
Colonic Neoplasms* / microbiology
Colorectal Neoplasms* / diet therapy
Colorectal Neoplasms* / microbiology
Dysbiosis* / diet therapy
Dysbiosis* / microbiology
Gastrointestinal Microbiome / drug effects*
Intestines / microbiology
Intestines / pathology
Male
Malus / metabolism
Mice
Mice, Inbred ICR
Polysaccharides / pharmacology*
Wnt Signaling Pathway

Substances

Polysaccharides