Human anthrax cases necessitate rapid response. We completed Bacillus anthracis nanopore whole-genome sequencing in our high-containment laboratory from a human anthrax isolate hours after receipt. The de novo assembled genome showed no evidence of known antimicrobial resistance genes or introduced plasmid(s). Same-day genomic characterization enhances public health emergency response.
Keywords: Bacillus anthracis; anthrax; bacteria; bioterrorism and preparedness; emergency preparedness; nanopore; whole-genome sequencing; zoonoses.