Acridinium benzoate was developed as a unique ICT-based fluorescent scaffold for both ratiometric and turn-on fluorescence imaging through decaging of the phenolic hydroxyl groups. Two fluorescent probes, Acr1-H2 O2 and Acr1-β-gal, were developed for the fluorescence imaging of H2 O2 and β-galactosidase in vivo.
Keywords: acridinium benzoates; fluorescein; fluorescence imaging; ratiometric sensing.
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