Hyperoside inhibited the migration and invasion of lung cancer cells through the upregulation of PI3K/AKT and p38 MAPK pathways

Non-small cell lung cancer (NSCLC) is one of the most common human malignancies. Malignant tumor is characterized by the Invasion and metastasis of tumor. Based on previous research, human A549 cells could be induced to apoptosis by Hyperoside (Hyp). The aim of the present study was to investigate whether low concentrations of Hyp (1-10 μM) could inhibit the invasion and migration of lung cancer cells through regulation of phosphoinositide-3-kinase/serine threonine kinase (PI3K/AKT) and p38 mitogen-activated protein kinase (p38 MAPK) pathways.CCK8 assay was performed to identify the cell viability after human A549 cells treated with Hyp (1, 2 and 5 μM) 12, 24 and 48 h. Hyp (1, 2 and 5 μM) significantly suppressed the invasion and migration of A549 cells in a dose-dependant manner. Phosphorylation of AKT and P38 protein level were measured by western blot. By RT-PCR and western blot analysis, non-metastatic gene23-H1 (nm23-H1), metastasis-associated gene 1 (MTA1), tissue inhibitor of metalloproteinase-1 (TIMP-2) and matrix metalloproteinases-2/9 (MMP-2/9) expression was estimated. The results showed that the level of nm23-H1, MTA1, TIMP-2 and MMP-2/9 expression were all regulated dramatically in Hyp groups compared with the control group. The p-ATK and p-P38 were descended notably. These results suggested that Hyp significantly suppressed the invasion and migration of A549 cells by mediating the AKT/PI3K and p38 MAPK pathways and regulated the expressions of invasion and migration related genes.