Electrochemiluminescence aptasensor for multiple determination of Hg2+ and Pb2+ ions by using the MIL-53(Al)@CdTe-PEI modified electrode

Anal Chim Acta. 2020 Mar 1:1100:232-239. doi: 10.1016/j.aca.2019.11.069. Epub 2019 Nov 30.

Abstract

An aptasensor based on MIL-53(Al)@CdTe was designed for multiple determination of Hg2+ and Pb2+ by electrochemiluminescence (ECL). Upon the recognition of Hg2+, aptamer 2-AuNPs form hairpin structures and are removed from the electrode. While in the presence of Pb2+, aptamer 1-PtNPs capture the target ions and form G-quadruplexes, and then bring PtNPs close enough to CdTe QDs to produce ECL resonance energy transfer. Upon aptamer interaction with Hg2+ and Pb2+, decreased ECL intensity was observed due to enhanced resonance energy transfer (ERET) and attenuated surface plasmon resonance (SPR). The ECL intensity difference (ΔECL) could therefore be used to detect heavy-metal ions with detection limits of 4.1 × 10-12 M (path 1, Hg2+), 3.7 × 10-11 M (path 2, Pb2+), and 2.4 × 10-11 M (path 3, Pb2+). The aptasensor could also be used for detecting Hg2+ and Pb2+ in fish and shrimp samples with good recoveries.

Keywords: ECL resonance energy transfer; Electrochemiluminescence; Heavy-metal ions; Metal-organic frameworks; Quantum dots.

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Biosensing Techniques*
  • Cadmium / chemistry
  • Electrochemical Techniques*
  • Electrodes
  • Ions / analysis
  • Lead / analysis*
  • Luminescence
  • Mercury / analysis*
  • Metal-Organic Frameworks / chemistry*
  • Particle Size
  • Polyethyleneimine / chemistry
  • Surface Properties
  • Tellurium / chemistry

Substances

  • Aptamers, Nucleotide
  • Ions
  • Metal-Organic Frameworks
  • Cadmium
  • Lead
  • Polyethyleneimine
  • Mercury
  • Tellurium