Abstract
A group of asymmetric Si-rhodamine scaffolds was designed for protease-activated NIR probes. Dual pH-inertia for both spirocyclized fluorescent probes and fluorescent products of zwitterions form over a wide range of pH (4.0-11.0). Leucine aminopeptidase (LAP) and γ-glutamyl transpeptidase (GGT) were monitored by fluorescent imaging in vivo.
MeSH terms
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry*
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Fluorescent Dyes / metabolism
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HeLa Cells
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Humans
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Hydrogen-Ion Concentration
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Infrared Rays
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Molecular Structure
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Optical Imaging*
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Peptide Hydrolases / analysis*
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Peptide Hydrolases / metabolism
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Rhodamines / chemistry*
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Rhodamines / metabolism
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Silicon / chemistry*
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Silicon / metabolism
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Spectrometry, Fluorescence
Substances
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Fluorescent Dyes
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Rhodamines
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Peptide Hydrolases
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Silicon