Abstract
Glucagon-like peptide-1 (GLP-1) was purified to homogeneity by HPLC and anion-exchange chromatography. A molecular mass of 3297.4 Da was obtained by FAB mass spectrometry which corresponded exactly to GLP-1 7-36 NH2, providing evidence that amidation occurs at an arginine residue during the post-translational processing of GLP-1. The distribution of GLP-1 7-36 NH2-like immunoreactivity (GLP-1 7-36 NH2 IR) was determined in the rat gastrointestinal tract. Highest concentrations were found in terminal ileum and colon. Streptozocin-induced diabetic rats, who showed a significant increase in food intake, had a significant increase of GLP-1 7-36 NH2 IR in the colon.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Chromatography, High Pressure Liquid
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Chromatography, Ion Exchange
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Colon / analysis
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Colon / metabolism
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Diabetes Mellitus, Experimental / metabolism*
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Eating
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Glucagon
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Glucagon-Like Peptide 1
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Glucagon-Like Peptides
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Ileum / analysis
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Ileum / metabolism
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Intestinal Mucosa / metabolism*
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Intestines / analysis
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Mass Spectrometry
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Molecular Weight
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Peptide Fragments*
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Peptides / analysis
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Peptides / isolation & purification
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Peptides / metabolism*
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Rats
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Rats, Inbred Strains
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Tissue Distribution
Substances
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Peptide Fragments
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Peptides
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glucagon-like peptide 1 (7-36)amide
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Glucagon-Like Peptides
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Glucagon-Like Peptide 1
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Glucagon