Biomineralized nanoparticles enable an enzyme-assisted DNA signal amplification in living cells

Chem Commun (Camb). 2020 Mar 5;56(19):2901-2904. doi: 10.1039/c9cc09503a.

Abstract

The enzymatic-assisted signal amplification of DNA sensors is rarely applied in living cells due to the difficulties in protein delivery. In this study, we have proposed a biomineralization-based DNA nanoprobe to transport nucleases and DNA sensors for enzyme-assisted imaging of microRNA in living cells.

MeSH terms

  • Biomineralization*
  • DNA / metabolism*
  • DNA Probes / chemistry*
  • Exodeoxyribonucleases / metabolism*
  • Humans
  • Metal-Organic Frameworks / chemistry
  • MicroRNAs / metabolism
  • Nanoparticles / chemistry*

Substances

  • DNA Probes
  • Metal-Organic Frameworks
  • MicroRNAs
  • DNA
  • Exodeoxyribonucleases
  • exodeoxyribonuclease III