Umbilical cord as an analytical matrix - A technical note

Hayley R Price; Camron Chehroudi; Stuart J Knight; Alexander D Smith; Dickson Lai; Hugh Kim; Tricia E Wright; Michael Wh Coughtrie; Abby C Collier

doi:10.1016/j.placenta.2019.12.001

Umbilical cord as an analytical matrix - A technical note

Placenta. 2020 Jan 15:90:42-44. doi: 10.1016/j.placenta.2019.12.001. Epub 2019 Dec 3.

Authors

Hayley R Price¹, Camron Chehroudi², Stuart J Knight¹, Alexander D Smith¹, Dickson Lai¹, Hugh Kim², Tricia E Wright³, Michael Wh Coughtrie¹, Abby C Collier⁴

Affiliations

¹ Faculty of Pharmaceutical Sciences, The University of British Columbia, 2405 Wesbrook Mall, Vancouver, BC, V6T 1Z3, Canada.
² Centre for Blood Research, 2350 Health Sciences Mall, The University of British Columbia, Vancouver, BC, V6T 1Z3, Canada; Faculty of Dentistry, 2199 Wesbrook Mall, The University of British Columbia, Vancouver, BC, V6T 1Z3, Canada; Department of Biochemistry and Molecular Biology, 2350 Health Sciences Mall, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada.
³ Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, San Francisco, CA, 94115, USA.
⁴ Faculty of Pharmaceutical Sciences, The University of British Columbia, 2405 Wesbrook Mall, Vancouver, BC, V6T 1Z3, Canada. Electronic address: [email protected].

PMID: 32056550
DOI: 10.1016/j.placenta.2019.12.001

Abstract

The umbilical cord (UC) connects the fetal blood supply to the placenta, so is exposed to all systemic endo- and xenobiotics. We have extensive experience using UC as an analytical matrix for detecting and/or quantitating drugs, chemicals and endogenous compounds. This technical note describes advantages (large amount available, ease of collection, small sample needed for use, rapid availability) and challenges (clinical relationships, processing difficulties, matrix effects on analytes and detection technologies) of UC as an analytical matrix in ELISA and LC/MS platforms, and provides guidance for successfully working with this tissue.

Keywords: Drug screening; ELISA; Laboratory methods; Mass spectrometry; Maternal-fetal outcomes; Pharmacokinetics; Toxicology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Drug Evaluation, Preclinical / methods*
Fetal Blood / chemistry
Humans
Mass Spectrometry*
Umbilical Cord / chemistry*

Abstract

Publication types

MeSH terms

Grants and funding