Molecular characterization of a novel fusarivirus infecting the plant-pathogenic fungus Botryosphaeria dothidea

Arch Virol. 2020 Apr;165(4):1033-1037. doi: 10.1007/s00705-020-04554-1. Epub 2020 Feb 17.

Abstract

A novel virus, Botryosphaeria dothidea fusarivirus 1 (BdFV1), was isolated from a fungal strain, SDAU11-86 of Botryosphaeria dothidea, and its complete genome sequence was determined. BdFV1 has a single-stranded positive-sense (+ssRNA) genome with 6,179 nucleotides, excluding the poly(A) tail. The genome of BdFV1 contains two putative open reading frames (ORFs). The first ORF encodes a large polyprotein of 1,544 amino acids (aa) with conserved RNA-dependent RNA polymerase and viral helicase domains. The second ORF encodes a putative 481-aa protein with unknown function. Sequence comparisons and phylogenetic analysis suggested that BdFV1 is a novel mycovirus belonging to the newly proposed family "Fusariviridae". This is the first report of a +ssRNA mycovirus in B. dothidea.

MeSH terms

  • Ascomycota / virology*
  • Fungal Viruses / classification
  • Fungal Viruses / genetics
  • Fungal Viruses / isolation & purification*
  • Genome, Viral
  • Open Reading Frames
  • Phylogeny
  • Plant Diseases / microbiology
  • Plants / virology
  • RNA Viruses / classification
  • RNA Viruses / genetics
  • RNA Viruses / isolation & purification*
  • Viral Proteins / genetics

Substances

  • Viral Proteins