Effect of TRF 1 rs3863242 polymorphism on telomere length in omethoate-exposed workers

J Environ Sci Health B. 2020;55(6):525-529. doi: 10.1080/03601234.2020.1728167. Epub 2020 Feb 20.

Abstract

Telomere length was found to be associated with omethoate exposure and polymorphisms in certain genes among occupational workers. However, whether the polymorphisms in telomere-binding protein genes influence telomere length remains unclear. To explore the correlation between telomere length and polymorphisms in telomere-binding protein genes, telomere length in peripheral blood leukocytes was determined by real-time quantitative polymerase chain reaction in 180 omethoate-exposed workers and 115 healthy controls. Polymorphisms in 10 pairs of alleles were detected using flight mass spectrometry or polymerase chain reaction-restriction fragment length polymorphism technique. The results showed that individuals with GG genotype in TRF1 rs3863242 had longer telomere lengths than those with AG + AA genotype in the control group (p = 0.005). The multiple regression analysis suggested that both omethoate exposure (b = 0.526, p < 0.001) and TRF1 rs3863242 GG (b = 0.220, p = 0.002) were related to a longer telomere length. In conclusion, GG genotype in TRF1 rs3863242 is linked to prolongation of telomere length, and individuals with GG genotype are recommended to strengthen health protection in a Chinese occupational omethoate-exposed population.

Keywords: Omethoate; TRF1 rs3863242; polymorphism; telomere length; telomere-binding protein genes.

MeSH terms

  • Adult
  • Asian People
  • Case-Control Studies
  • Dimethoate / analogs & derivatives*
  • Dimethoate / toxicity
  • Female
  • Genotype
  • Humans
  • Male
  • Middle Aged
  • Occupational Exposure* / adverse effects
  • Polymerase Chain Reaction / methods
  • Polymorphism, Restriction Fragment Length
  • Polymorphism, Single Nucleotide*
  • Shelterin Complex
  • Telomere / drug effects*
  • Telomere-Binding Proteins / genetics
  • Telomeric Repeat Binding Protein 1 / genetics*
  • Telomeric Repeat Binding Protein 2 / genetics

Substances

  • POT1 protein, human
  • Shelterin Complex
  • TERF2 protein, human
  • Telomere-Binding Proteins
  • Telomeric Repeat Binding Protein 1
  • Telomeric Repeat Binding Protein 2
  • dimethoxon
  • Dimethoate