Abstract
An Escherichia coli strain isolated from the feces of swine at a pork slaughterhouse in Henan province China was found to possess two colistin-resistance genes, mcr-1 and mcr-3, plus 16 additional resistance genes. Genes mcr-1.1 and mcr-3.1 were identified on IncHI2 and IncX1 type plasmids, respectively. Transconjugants (containing mcr-3, mcr-1&mcr-3) were obtained that were 64- and 512-fold higher than the minimum inhibitory concentration of colistin on the recipient bacteria (E. coli C600), respectively. The IncX1 plasmid containing mcr-3.1 displayed a very specific structure compared with previous mcr-3. Variable and stable regions were similar across different plasmids, multiple insertion sequences and transposases.
Keywords:
InX1 plasmid; colistin; mcr-1.1; mcr-3.1; multidrug resistance; swine.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Bacterial Agents / metabolism
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Anti-Bacterial Agents / pharmacology*
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China
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Colistin / pharmacology
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DNA, Bacterial
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Drug Resistance, Multiple, Bacterial*
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Escherichia coli / drug effects*
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Escherichia coli / genetics*
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Escherichia coli / isolation & purification
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Escherichia coli / metabolism
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Escherichia coli Infections / microbiology
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Escherichia coli Proteins / genetics*
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Escherichia coli Proteins / metabolism
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Feces / microbiology
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Interspersed Repetitive Sequences
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Microbial Sensitivity Tests
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Plasmids
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Swine
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Transferases (Other Substituted Phosphate Groups) / genetics*
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Transferases (Other Substituted Phosphate Groups) / metabolism
Substances
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Anti-Bacterial Agents
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DNA, Bacterial
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Escherichia coli Proteins
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MCR-1 protein, E coli
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MCR-3 protein, E coli
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Transferases (Other Substituted Phosphate Groups)
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Colistin