Comparison of the Efficacy and Sensitivity of Alternative PET Reporter Gene/PET Reporter Probe Systems That Minimize Biological Variables

Shili Xu; Harvey R Herschman

doi:10.1007/978-1-0716-0364-2_16

Comparison of the Efficacy and Sensitivity of Alternative PET Reporter Gene/PET Reporter Probe Systems That Minimize Biological Variables

Methods Mol Biol. 2020:2126:177-190. doi: 10.1007/978-1-0716-0364-2_16.

Authors

Shili Xu¹, Harvey R Herschman^{2

3

4

5

6}

Affiliations

¹ Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.
² Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA. [email protected].
³ Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA. [email protected].
⁴ Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA. [email protected].
⁵ Department of Biological Chemistry, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA. [email protected].
⁶ Molecular Biology Institute, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA. [email protected].

PMID: 32112389
DOI: 10.1007/978-1-0716-0364-2_16

Abstract

Positron emission tomography (PET) reporter genes (PRGs), when coupled with positron-emitting PET reporter probes (PRPs), are useful for tracking specific cell populations in cell-based therapies, in transgenic animal models, and in xenograft tumor progression experiments. The activities of incorporated PRGs in targeted cells can be monitored noninvasively by PET imaging in preclinical in vivo studies and clinical applications following systemic administration of the appropriate PRG. Here we describe a method that minimizes both design and variability of vector delivery vehicles for alternative PRGs and biological variability of the in vivo target when comparing the efficacy, sensitivity, and specificity of alternative PRG/PRP combinations for in vivo PRG imaging. The principles described for comparing alternative PRG/PRP reporter gene systems can be applied to comparisons of alternative fluorescence, bioluminescence, single-photon emission computerized tomography (SPECT), and magnetic resonance imaging (MRI) reporter genes.

Keywords: Adenovirus; PET reporter genes; PET reporter probes; Positron emission tomography; Reporter gene imaging.

Publication types

Comparative Study

MeSH terms

Adenoviridae / genetics*
Animals
Female
Genes, Reporter*
Humans
Male
Mice
Mice, Inbred NOD
Mice, SCID
Molecular Imaging / methods*
Positron-Emission Tomography / methods*
Thymidine Kinase / analysis*

Substances

Thymidine Kinase