Lipopolysaccharide-dependent enhancement of adherence-mediated chemiluminescence response of polymorphonuclear leukocytes

J Biolumin Chemilumin. 1988 Jan-Mar;2(1):35-9. doi: 10.1002/bio.1170020107.

Abstract

Adherence of resting polymorphonuclear leukocytes to nylon fibre increased the chemiluminescence response (CL) from 99,400 to 910,300 cpm/25,000 PMNL. This effect could be amplified by lipopolysaccharide (LPS) priming of granulocytes in a dose-dependent fashion. The results of nylon fibre adherence experiments suggest an in vitro model that might approximate certain conditions of in vivo PMNL-endothelial adherence and respiratory burst activation, and these reactions of polymorphonuclear leukocytes may contribute to the pathomechanisms of the Adult Respiratory Distress Syndrome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Adhesion / drug effects
  • Granulocytes / cytology
  • Granulocytes / physiology
  • Humans
  • Kinetics
  • Lipopolysaccharides / pharmacology*
  • Luminescent Measurements*
  • Neutrophils / cytology
  • Neutrophils / drug effects
  • Neutrophils / physiology*
  • Nylons

Substances

  • Lipopolysaccharides
  • Nylons