Integration of Whole-Genome Sequencing With Circulating Tumor DNA Analysis Captures Clonal Evolution and Tumor Heterogeneity in Non-V600 BRAF Mutant Colorectal Cancer

Shehara Mendis; Miguel Alcaide; James T Topham; Benny Johnson; Ryan D Morin; Jenny Chu; Ian Bosdet; Scott Kopetz; Aly Karsan; Sharlene Gill; Janessa Laskin; Steven J M Jones; Marco A Marra; David F Schaeffer; Daniel J Renouf; Jonathan M Loree

doi:10.1016/j.clcc.2020.02.001

Integration of Whole-Genome Sequencing With Circulating Tumor DNA Analysis Captures Clonal Evolution and Tumor Heterogeneity in Non-V600 BRAF Mutant Colorectal Cancer

Clin Colorectal Cancer. 2020 Jun;19(2):132-136.e3. doi: 10.1016/j.clcc.2020.02.001. Epub 2020 Feb 10.

Authors

Shehara Mendis¹, Miguel Alcaide², James T Topham³, Benny Johnson⁴, Ryan D Morin⁵, Jenny Chu⁶, Ian Bosdet⁷, Scott Kopetz⁴, Aly Karsan⁷, Sharlene Gill¹, Janessa Laskin¹, Steven J M Jones⁸, Marco A Marra⁸, David F Schaeffer⁹, Daniel J Renouf¹⁰, Jonathan M Loree¹¹

Affiliations

¹ Medical Oncology, BC Cancer, Vancouver, British Columbia, Canada.
² Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada.
³ Pancreas Centre BC, Vancouver, British Columbia, Canada.
⁴ Department of Gastrointestinal Medical Oncology, The University of M.D. Anderson Cancer Center, Houston, TX.
⁵ Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
⁶ Department of Pathology and Laboratory Medicine, Vancouver General Hospital, Vancouver, British Columbia, Canada.
⁷ Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
⁸ Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada; Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada.
⁹ Pancreas Centre BC, Vancouver, British Columbia, Canada; Department of Pathology and Laboratory Medicine, Vancouver General Hospital, Vancouver, British Columbia, Canada.
¹⁰ Medical Oncology, BC Cancer, Vancouver, British Columbia, Canada; Pancreas Centre BC, Vancouver, British Columbia, Canada.
¹¹ Medical Oncology, BC Cancer, Vancouver, British Columbia, Canada. Electronic address: [email protected].

PMID: 32151517
DOI: 10.1016/j.clcc.2020.02.001

No abstract available

Keywords: Anti-epidermal growth factor receptor; Colon; Colorectal neoplasms; EGFR; ctDNA.

Publication types

Case Reports
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Antineoplastic Combined Chemotherapy Protocols / therapeutic use
Biomarkers, Tumor / blood
Biomarkers, Tumor / genetics*
Biopsy
Circulating Tumor DNA / blood
Circulating Tumor DNA / genetics*
Clonal Evolution
Colorectal Neoplasms / blood
Colorectal Neoplasms / drug therapy
Colorectal Neoplasms / genetics*
Colorectal Neoplasms / pathology
ErbB Receptors / antagonists & inhibitors
Genetic Heterogeneity
Humans
Liver / pathology
Liver Neoplasms / diagnosis
Liver Neoplasms / drug therapy
Liver Neoplasms / genetics*
Liver Neoplasms / secondary
Male
Mutation
Proto-Oncogene Proteins B-raf / genetics*
Whole Genome Sequencing

Substances

Biomarkers, Tumor
Circulating Tumor DNA
EGFR protein, human
ErbB Receptors
BRAF protein, human
Proto-Oncogene Proteins B-raf