Generation of a male common marmoset embryonic stem cell line DSY127-BV8VT1 carrying double reporters specific for the germ cell linage using the CRISPR-Cas9 and PiggyBac transposase systems

Sho Yoshimatsu; Tsukika Sato; Masafumi Yamamoto; Erika Sasaki; Mayutaka Nakajima; Mari Nakamura; Seiji Shiozawa; Toshiaki Noce; Hideyuki Okano

doi:10.1016/j.scr.2020.101740

Generation of a male common marmoset embryonic stem cell line DSY127-BV8VT1 carrying double reporters specific for the germ cell linage using the CRISPR-Cas9 and PiggyBac transposase systems

Stem Cell Res. 2020 Apr:44:101740. doi: 10.1016/j.scr.2020.101740. Epub 2020 Feb 21.

Authors

Sho Yoshimatsu¹, Tsukika Sato², Masafumi Yamamoto³, Erika Sasaki⁴, Mayutaka Nakajima², Mari Nakamura², Seiji Shiozawa², Toshiaki Noce⁵, Hideyuki Okano⁶

Affiliations

¹ Department of Physiology, School of Medicine, Keio University, Tokyo, Japan; Laboratory for Proteolytic Neuroscience, RIKEN Center for Brain Science, Saitama, Japan. Electronic address: [email protected].
² Department of Physiology, School of Medicine, Keio University, Tokyo, Japan.
³ ICLAS Monitoring Center, Central Institute for Experimental Animals, Kanagawa, Japan.
⁴ Department of Marmoset Biology and Medicine, Central Institute for Experimental Animals, Kanagawa, Japan.
⁵ Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan.
⁶ Department of Physiology, School of Medicine, Keio University, Tokyo, Japan; Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan. Electronic address: [email protected].

PMID: 32151954
DOI: 10.1016/j.scr.2020.101740

Abstract

BLIMP1 (PRDM1) and VASA (DDX4) play pivotal roles in the development of the germ cell linage. Importantly, these genes are specifically expressed in germ cells; BLIMP1 in primordial germ cells (PGCs) to early-stage gonocytes, and VASA in migration-stage PGCs to mature gametes. The high reproductive efficiency of common marmosets (marmosets; Callithrix jacchus) makes them advantageous for use in germ cell research. We herein report the generation of a male marmoset embryonic stem cell (ESC) line harboring BLIMP1 and DDX4 double reporters. This ESC line will be a useful tool for investigating male gametogenesis in non-human primates.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CRISPR-Cas Systems / genetics
Callithrix*
Cell Differentiation
Cell Line*
Embryonic Stem Cells*
Germ Cells
Male
Transposases*

Substances

Transposases