Irisin lowers blood pressure by activating the Nrf2 signaling pathway in the hypothalamic paraventricular nucleus of spontaneously hypertensive rats

Toxicol Appl Pharmacol. 2020 May 1:394:114953. doi: 10.1016/j.taap.2020.114953. Epub 2020 Mar 10.

Abstract

Exercise training is one of the major non-pharmacological treatments for hypertension. However, the central mechanism by which exercise training attenuates the hypertensive responses remains unclear. Irisin is a muscle-secreted cytokine derived from fibronectin type III domain containing 5 (FNDC5) that will be released into the circulation during exercise. We hypothesized that irisin may play a role in the blood pressure regulation by exercise. To examine the hypothesis, our study investigated the effect of irisin on hypertension and its central mechanism. The study was performed in spontaneously hypertensive rats (SHRs) and normotensive Wistar-Kyoto rats. We found that intravenous injection of irisin effectively reduced blood pressure, plasma norepinephrine, paraventricular nucleus (PVN) levels of neuronal activation, oxidative stress and inflammation in SHRs. Moreover, irisin activated nuclear factor E2-related factor-2 (Nrf2) and restored the imbalance of neurotransmitters in the PVN. Our study also found PVN knockdown of Nrf2 abolished the protective effects of irisin on hypertension. These findings demonstrate irisin can improve hypertension via Nrf2-mediated antioxidant in the PVN.

Keywords: Hypertension; Irisin; Nrf2; Oxidative stress; Paraventricular nucleus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antihypertensive Agents / pharmacology*
  • Antioxidants / metabolism
  • Cytokines / metabolism
  • Fibronectins / pharmacology*
  • NF-E2-Related Factor 2 / drug effects*
  • Neurotransmitter Agents / metabolism
  • Norepinephrine / blood
  • Oxidative Stress / drug effects
  • Paraventricular Hypothalamic Nucleus / drug effects*
  • Physical Exertion
  • Rats
  • Rats, Inbred SHR
  • Rats, Inbred WKY
  • Signal Transduction / drug effects*

Substances

  • Antihypertensive Agents
  • Antioxidants
  • Cytokines
  • FNDC5 protein, rat
  • Fibronectins
  • NF-E2-Related Factor 2
  • Neurotransmitter Agents
  • Nfe2l2 protein, rat
  • Norepinephrine