Transgenerational plasticity of inducible defences: Combined effects of grand-parental, parental and current environments

Phenotypic plasticity can occur across generations (transgenerational plasticity) when environments experienced by the previous generations influenced offspring phenotype. The evolutionary importance of transgenerational plasticity, especially regarding within-generational plasticity, is a currently hot topic in the plasticity framework. How long an environmental effect can persist across generations and whether multigenerational effects are cumulative are primordial-for the evolutionary significance of transgenerational plasticity-but still unresolved questions. In this study, we investigated how the grand-parental, parental and offspring exposures to predation cues shape the predator-induced defences of offspring in the Physa acuta snail. We expected that the offspring phenotypes result from a three-way interaction among grand-parental, parental and offspring environments. We exposed three generations of snails without and with predator cues according to a full factorial design and measured offspring inducible defences. We found that both grand-parental and parental exposures to predator cues impacted offspring antipredator defences, but their effects were not cumulative and depended on the defences considered. We also highlighted that the grand-parental environment did alter reaction norms of offspring shell thickness, demonstrating an interaction between the grand-parental transgenerational plasticity and the within-generational plasticity. We concluded that the effects of multigenerational exposure to predator cues resulted on complex offspring phenotypic patterns which are difficult to relate to adaptive antipredator advantages.