Data on analysis of OCC-1 transcript levels in pluripotent and differentiated states of P19 cells

Zahra Hosseininia; Sara Soltanian; Naser Mahdavi-Shahri; Hesam Dehghani

doi:10.1016/j.dib.2020.105367

Data on analysis of OCC-1 transcript levels in pluripotent and differentiated states of P19 cells

Data Brief. 2020 Feb 29:29:105367. doi: 10.1016/j.dib.2020.105367. eCollection 2020 Apr.

Authors

Zahra Hosseininia^{1

2}, Sara Soltanian³, Naser Mahdavi-Shahri⁴, Hesam Dehghani^{1

2}

Affiliations

¹ Division of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
² Stem Cells and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
³ Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
⁴ Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.

Abstract

We investigated the expression of OCC-1 at mRNA level during retinoic acid (RA) induced differentiation of mouse embryonic carcinoma P19 pluripotent cancer cells by quantitative real time PCR (qPCR). By employing four-fold serial dilutions of P19 cDNA, standard curves were generated for the reference gene (L37) and the gene of interest (OCC-1). PCR efficiencies for L37 and OCC-1 were calculated. Since the amplification efficiencies of these two genes were unequal, the standard curve method was used for the relative quantification of OCC-1. Data analysis revealed that the expression of OCC-1 was reduced by about 69% after 4-day treatment with RA, when significant down-regulation of key pluripotency factors, including OCT4 and Nanog was observed [1].

Keywords: Differentiation; OCC-1; P19; Pluripotency; Retinoic acid.