Rb analog Whi5 regulates G1 to S transition and cell size but not replicative lifespan in budding yeast

Matthew M Crane; Mitsuhiro Tsuchiya; Ben W Blue; Jared D Almazan; Kenneth L Chen; Siobhan R Duffy; Alexandra Golubeva; Annaiz M Grimm; Alison M Guard; Shauna A Hill; Ellen Huynh; Ryan M Kelly; Michael Kiflezghi; Hyunsung D Kim; Mitchell Lee; Ting-I Lee; Jiayi Li; Bao M G Nguyen; Riley M Whalen; Feng Y Yeh; Mark McCormick; Brian K Kennedy; Joe R Delaney; Matt Kaeberlein

doi:10.1016/j.tma.2019.10.002

Rb analog Whi5 regulates G1 to S transition and cell size but not replicative lifespan in budding yeast

Transl Med Aging. 2019:3:104-108. doi: 10.1016/j.tma.2019.10.002. Epub 2019 Oct 31.

Authors

Matthew M Crane¹, Mitsuhiro Tsuchiya¹, Ben W Blue¹, Jared D Almazan¹, Kenneth L Chen^{1

2

3}, Siobhan R Duffy¹, Alexandra Golubeva¹, Annaiz M Grimm¹, Alison M Guard¹, Shauna A Hill¹, Ellen Huynh¹, Ryan M Kelly¹, Michael Kiflezghi¹, Hyunsung D Kim¹, Mitchell Lee¹, Ting-I Lee¹, Jiayi Li¹, Bao M G Nguyen¹, Riley M Whalen¹, Feng Y Yeh¹, Mark McCormick⁴, Brian K Kennedy⁵, Joe R Delaney⁶, Matt Kaeberlein^{1

2}

Affiliations

¹ Department of Pathology, University of Washington, Seattle, WA, USA.
² Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
³ Medical Scientist Training Program, University of Washington, Seattle, Washington USA.
⁴ Department of Biochemistry and Molecular Biology, University of New Mexico, Albuquerque, NM, USA.
⁵ Buck Institute for Research on Aging, Novato, CA, USA.
⁶ Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC, USA.

Abstract

An increase in cell size with age is a characteristic feature of replicative aging in budding yeast. Deletion of the gene encoding Whi5 results in shortened duration of G1 and reduced cell size, and has been previously suggested to increase replicative lifespan. Upon careful analysis of multiple independently derived haploid and homozygous diploid whi5Δ mutants, we find no effect on lifespan, but we do confirm the reduction in cell size. We suggest that instead of antagonizing lifespan, the elongated G1 phase of the cell cycle during aging may actually play an important role in allowing aged cells time to repair accumulating DNA damage.

Abstract

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