Purification and identification of antioxidant peptides from duck plasma proteins

Food Chem. 2020 Jul 30:319:126534. doi: 10.1016/j.foodchem.2020.126534. Epub 2020 Mar 2.

Abstract

The antioxidant peptides extracted from duck plasma hydrolysate (DPH) was investigated. The antioxidant activity of DPH, which was isolated and purified via ultrafiltration, size exclusion chromatography, and reversed-phase high-performance liquid chromatography, was evaluated using its free radical scavenging ability. Nano-liquid chromatography-tandem mass spectrometry was conducted to identify the DPH fractions with the highest antioxidant ability. Seven novel peptides: LDGP, TGVGTK, EVGK, RCLQ, LHDVK, KLGA, and AGGVPAG (400.43, 561.63, 431.48, 260.14, 610.71, 387.47, and 527.57 Da, respectively) were identified and synthesized using a solid-phase peptide produce to evaluate their antioxidant activities. Of these, EVGK exhibited the highest Fe2+ chelating ability (16.35%), and RCLQ presented the highest reducing power, 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt scavenging activity, and 1,1-diphenyl-2-picrylhydrazyl scavenging rate (0.62, 274.83 mM TE/mg, and 95.12%, respectively). Our results indicated that DPH possessed antioxidant capabilities and could be used to obtain antioxidant peptides, thus adding economic value to duck blood.

Keywords: Antioxidant activity; Antioxidant peptides; Duck plasma hydrolysate; Nano-liquid chromatography–tandem mass spectrometry; Separation and purification.

MeSH terms

  • Animals
  • Antioxidants / chemistry*
  • Antioxidants / isolation & purification
  • Biphenyl Compounds
  • Blood Proteins / chemistry*
  • Blood Proteins / isolation & purification
  • Chromatography, Gel
  • Ducks*
  • Peptides / chemistry*
  • Peptides / isolation & purification
  • Protein Hydrolysates / chemistry

Substances

  • Antioxidants
  • Biphenyl Compounds
  • Blood Proteins
  • Peptides
  • Protein Hydrolysates
  • biphenyl