Quantitative phase imaging of stromal prognostic markers in pancreatic ductal adenocarcinoma

Michael Fanous; Adib Keikhosravi; Andre Kajdacsy-Balla; Kevin W Eliceiri; Gabriel Popescu

doi:10.1364/BOE.383242

Quantitative phase imaging of stromal prognostic markers in pancreatic ductal adenocarcinoma

Biomed Opt Express. 2020 Feb 12;11(3):1354-1364. doi: 10.1364/BOE.383242. eCollection 2020 Mar 1.

Authors

Michael Fanous^{1

2}, Adib Keikhosravi³, Andre Kajdacsy-Balla⁴, Kevin W Eliceiri^{3

5}, Gabriel Popescu^{1

2}

Affiliations

¹ Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
² Quantitative Light Imaging Laboratory, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
³ Laboratory for Optical and Computational Instrumentation, Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA.
⁴ Department of Pathology, University of Illinois at Chicago, Chicago, IL 61801, USA.
⁵ Department of Medical Physics, University of Wisconsin, Madison, WI 53706, USA.

Abstract

New quantitative prognostic markers are needed for improved pancreatic ductal adenocarcinoma (PDAC) prognosis. Second harmonic generation microscopy has been used to show that collagen fiber alignment in PDAC is a negative prognostic factor. In this work, a series of PDAC and normal adjacent tissue (NAT) biopsies were imaged with spatial light interference microscopy (SLIM). Quantitative analysis performed on the biopsy SLIM images show that PDAC fiber structures have lower alignment per unit length, narrower width, and are longer than NAT controls. Importantly, fibrillar collagen in PDAC shows an inverse relationship between survival data and fiber width and length (p < 0.05).

Abstract

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