AhHDA1-mediated AhGLK1 promoted chlorophyll synthesis and photosynthesis regulates recovery growth of peanut leaves after water stress

Peanut (Arachis hypogaea L.) is an important crop that is adversely affected by drought. Post-drought growth is essential for improving peanut productivity and quality. Previous studies demonstrated that AhGLK1 (Arachis hypogaea L. Golden2-like 1) activates the expression of AhPORA to stimulate chlorophyll biosynthesis, and that AhGLK1 physically interacts with AhHDA1 (Arachis hypogaea L. histone deacetylase 1). However, the roles of the AhGLK1/AhHDA1 interaction in post-drought recovery remain to be elucidated. Herein, we report that AhHDA1 binds to AhGLK1 promoter and alters histone deacetylation levels to inhibit AhGLK1 expression. RNA-seq confirms that chlorophyll synthesis and photosynthesis-related genes are induced in AhGLK1-overexpressing, but reduced in AhGLK1 RNAi hairy roots. Furthermore, ChIP-seq shows that AhCAB (Arachis hypogaea L. chlorophyll A/B binding protein) is a target of both AhHDA1 and AhGLK1. Transactivation assays reveal that AhGLK1 activates AhCAB expression, while AhHDA1 inhibits the effect of AhGLK1 on AhCAB and AhPORA transcription. ChIP-qPCR shows that AhHDA1 and AhGLK1 bind to the promoters of AhCAB and AhPORA to regulate their expression during water stress and recovery. We propose that AhHDA1 and AhGLK1 consist of an ON/OFF switch for AhCAB and AhPORA expression during water stress and recovery. AhGLK1 activates, whereas AhHDA1 suppresses the expression of AhCAB and AhPORA.