Improved procedure for the construction of neoglycolipids having antigenic and lectin-binding activities, from reducing oligosaccharides

Biochem J. 1988 Dec 1;256(2):661-4. doi: 10.1042/bj2560661.

Abstract

Conditions have been established for the rapid and efficient conjugation of reducing oligosaccharides (di- to deca-saccharides) to dipalmitoyl phosphatidylethanolamine. The resulting neoglycolipids derived from several naturally occurring oligosaccharides and a series of N-linked high-mannose-type oligosaccharides released by hydrazinolysis from RNAase B showed specific and potent reactivities, as appropriate, with monoclonal antibodies to blood group Lewis(b), blood group A or a stage-specific embryonic (SSEA-1) antigen, or the lectin concanavalin A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amination
  • Antibody Specificity
  • Concanavalin A
  • Glycolipids / chemical synthesis*
  • Glycolipids / immunology
  • Humans
  • Molecular Probes / chemical synthesis
  • Oligosaccharides*
  • Phosphatidylethanolamines*

Substances

  • Glycolipids
  • Molecular Probes
  • Oligosaccharides
  • Phosphatidylethanolamines
  • Concanavalin A
  • 1,2-dipalmitoyl-3-phosphatidylethanolamine