The use of PCR and BsmAI restriction combination targeting wciP gene to determine serotype 6A, 6B, 6C and 6D Streptococcus pneumoniae

Wisnu Tafroji; Felicia Monica Bernadette; Ernawati A Giri Rachman; Dodi Safari

doi:10.1016/j.mimet.2020.105909

The use of PCR and BsmAI restriction combination targeting wciP gene to determine serotype 6A, 6B, 6C and 6D Streptococcus pneumoniae

J Microbiol Methods. 2020 May:172:105909. doi: 10.1016/j.mimet.2020.105909. Epub 2020 Apr 1.

Authors

Wisnu Tafroji¹, Felicia Monica Bernadette², Ernawati A Giri Rachman², Dodi Safari³

Affiliations

¹ Molecular Bacteriology Laboratory, Eijkman Institute for Molecular Biology, Indonesia.
² School of Life Sciences and Technology (SITH), Bandung Institute of Technology (ITB), Indonesia.
³ Molecular Bacteriology Laboratory, Eijkman Institute for Molecular Biology, Indonesia. Electronic address: [email protected].

PMID: 32246952
DOI: 10.1016/j.mimet.2020.105909

Abstract

We designed a sensitive and reliable method to distinguish serogroup 6 using PCR followed by enzymatic restriction digest. We discovered that this serotyping method was able to distinguish serotypes 6A, 6B, 6C, and 6D based on the recognition site of BsmAI in the wciP region.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques / methods
Deoxyribonucleases, Type II Site-Specific / metabolism*
Genes, Bacterial / genetics*
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Sequence Analysis, DNA
Serogroup
Serotyping / methods*
Streptococcus pneumoniae / classification*
Streptococcus pneumoniae / genetics*
Streptococcus pneumoniae / isolation & purification*

Substances

endodeoxyribonuclease BsmAI
Deoxyribonucleases, Type II Site-Specific