Sustained-release ketamine-loaded nanoparticles fabricated by sequential nanoprecipitation

Int J Pharm. 2020 May 15:581:119291. doi: 10.1016/j.ijpharm.2020.119291. Epub 2020 Apr 4.

Abstract

Ketamine in sub-anaesthetic doses is an analgesic adjuvant with a morphine-sparing effect. Co-administration of a strong opioid with an analgesic adjuvant such as ketamine is a potential treatment option, especially for patients with cancer-related pain. A limitation of ketamine is its short in vivo elimination half-life. Hence, our aim was to develop biocompatible and biodegradable ketamine-loaded poly(ethylene glycol) (PEG)-block-poly(lactic-co-glycolic acid) (PLGA) nanoparticles for sustained release. Ketamine-encapsulated single polymer PEG-PLGA nanoparticles and double polymer PEG-PLGA/shellac (SH) nanoparticles with a high drug loading of 41.8% (drug weight/the total weight of drug-loaded nanoparticles) were prepared using a new sequential nanoprecipitation method. These drug-loaded nanoparticles exhibited a sustained-release profile for up to 21 days in vitro and for more than 5 days after intravenous injection in mice. Our study demonstrates that high drug loading and a sustained release profile can be achieved with ketamine-loaded PEG-PLGA nanoparticles prepared using this new nanoprecipitation method.

Keywords: Drug delivery; High drug loading; Mice; PLGA; Pharmacokinetics.

MeSH terms

  • Analgesics / administration & dosage
  • Analgesics / chemistry
  • Analgesics / metabolism
  • Animals
  • Chemical Precipitation*
  • Delayed-Action Preparations / administration & dosage
  • Delayed-Action Preparations / chemical synthesis
  • Delayed-Action Preparations / metabolism
  • Drug Carriers / administration & dosage
  • Drug Carriers / chemical synthesis*
  • Drug Carriers / metabolism
  • Ketamine / administration & dosage
  • Ketamine / chemical synthesis*
  • Ketamine / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Nanoparticles / administration & dosage
  • Nanoparticles / chemistry*
  • Nanoparticles / metabolism
  • Polylactic Acid-Polyglycolic Acid Copolymer / administration & dosage
  • Polylactic Acid-Polyglycolic Acid Copolymer / chemical synthesis*
  • Polylactic Acid-Polyglycolic Acid Copolymer / metabolism
  • Tissue Distribution / drug effects
  • Tissue Distribution / physiology

Substances

  • Analgesics
  • Delayed-Action Preparations
  • Drug Carriers
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Ketamine