Abstract
Methods for the focused isolation of low-abundance natural products with specific chemical substructures could expand known bioactive chemical diversity for drug discovery. Here we report the combined use of genome mining and an 15N NMR-based screening method for the targeted isolation of the low-abundance piperazic-acid-containing peptides incarnatapeptins A (1) and B (3). Incarnatapeptin B (3) shows in vitro cytotoxicity to LNCaP prostate cancer cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / isolation & purification
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Antineoplastic Agents / pharmacology*
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Biological Products / chemistry
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Biological Products / isolation & purification
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Biological Products / pharmacology*
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Cell Survival / drug effects
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Drug Screening Assays, Antitumor
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Humans
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Magnetic Resonance Spectroscopy
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Nitrogen Isotopes
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Protons
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Streptomyces / chemistry*
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Streptomyces / genetics
Substances
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Antineoplastic Agents
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Biological Products
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Nitrogen Isotopes
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Nitrogen-15
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Protons