Gender differences on oxidative stress markers and complement component C3 plasma values after an oral unsaturated fat load test

Clin Investig Arterioscler. 2020 May-Jun;32(3):87-93. doi: 10.1016/j.arteri.2019.11.002. Epub 2020 Apr 11.
[Article in English, Spanish]

Abstract

Objective: Post-prandial lipaemia (PL), oxidative stress (OS), and complement component C3 (C3) values are related to the atherosclerosis process. The post-prandial response of C3 after an oral fat load test (OFLT) using unsaturated fat is poorly addressed. The aim of this study was to analyze and compare the post-prandial response of OS markers and C3 values in men and women after an OFLT using unsaturated fat.

Methods: The study included a total of 22 healthy subjects with normal lipids and normal blood glucose (11 men and 11 pre-menopausal women). An oral unsaturated fat load test (OFLT: 50g fat per m2 body surface) was performed using a commercial liquid preparation of long chain triglycerides (Supracal®). OS markers and C3 were measured using standardized methods at fasting state and every 2h up to 8h after the OFLT.

Results: Men showed statistically significant higher C3, oxidized glutathione (GSSG), and oxidized-reduced glutathione (GSSG/GSH) ratio values at fasting state compared to that obtained in women. In addition, post-prandial C3 values and GSSG/GSH ratios were significantly higher in men compared to women. The GSSG value and GSSG/GSH ratio significantly decreased in men after the OFLT compared to fasting values. In contrast, the post-prandial OS markers decrease observed in women was not statistically significant.

Conclusions: In fasting state, men showed higher statistically significant C3 values and OS markers than women. The post-prandial OS markers (GSSG and GSSG/GSH ratio) significantly decrease after the OFLT with unsaturated fat in men compared to women.

Keywords: Complement component C3; Componente del complemento C3; Estrés oxidativo; Grasa insaturada; Lipemia postprandial; Oral fat load test; Oxidative stress; Post-prandial lipaemia; Sobrecarga oral grasa; Unsaturated fat.

MeSH terms

  • Adult
  • Biomarkers / metabolism
  • Complement C3 / metabolism*
  • Fasting / physiology
  • Fats, Unsaturated / administration & dosage*
  • Female
  • Glutathione / metabolism
  • Glutathione Disulfide / metabolism
  • Humans
  • Lipids / blood*
  • Male
  • Middle Aged
  • Oxidative Stress / physiology*
  • Postprandial Period
  • Sex Factors
  • Triglycerides / administration & dosage

Substances

  • Biomarkers
  • Complement C3
  • Fats, Unsaturated
  • Lipids
  • Triglycerides
  • Glutathione
  • Glutathione Disulfide