Cord Blood Serum (CBS)-Based Eye Drops Modulate Light-Induced Neurodegeneration in Albino Rat Retinas

Biomolecules. 2020 Apr 28;10(5):678. doi: 10.3390/biom10050678.

Abstract

Age-related macular degeneration (AMD) is one of the leading causes of visual loss in western countries, it has no cure, and its incidence will grow in the future, for the overall population aging. Albino rats with retinal degeneration induced by exposure to high-intensity light (light-damage, LD) have been extensively used as a model of AMD to test neuroprotective agents. Among them, trophic factors (NGF and BDNF) have been shown to play a significant role in photoreceptors' survival. Interestingly, cord blood serum (CBS) is an extract full of chemokines and trophic factors; we, therefore, hypothesized that CBS could be an excellent candidate for neuroprotection. Here, we investigate whether CBS-based eye drops might mitigate the effects of light-induced retinal degeneration in albino rats. CBS treatment significantly preserved flash-electroretinogram (f-ERG) response after LD and reduced the "hot-spot" extension. Besides, CBS-treated animals better preserved the morphology of the outer nuclear layer, together with a reduction in microglia migration and activation. Interestingly, the treatment did not modulate reactive gliosis and activation of the self-protective mechanism (FGF2). In conclusion, our results suggest that CBS-based eye drops might be successfully used to mitigate retinal neurodegenerative processes such as AMD.

Keywords: cord blood serum; photoreceptor neurodegeneration; retina; trophic factor.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Epidermal Growth Factor / analysis
  • Epidermal Growth Factor / pharmacology
  • Female
  • Fetal Blood / chemistry*
  • Humans
  • Interleukins / analysis
  • Interleukins / pharmacology
  • Light / adverse effects
  • Macular Degeneration / drug therapy*
  • Macular Degeneration / etiology
  • Microglia / drug effects
  • Nerve Growth Factors / analysis
  • Nerve Growth Factors / pharmacology
  • Neuroprotective Agents / chemistry
  • Neuroprotective Agents / pharmacology*
  • Neuroprotective Agents / therapeutic use
  • Ophthalmic Solutions / chemistry
  • Ophthalmic Solutions / pharmacology*
  • Ophthalmic Solutions / therapeutic use
  • Photoreceptor Cells / drug effects*
  • Rats
  • Rats, Sprague-Dawley
  • Serum / chemistry

Substances

  • Interleukins
  • Nerve Growth Factors
  • Neuroprotective Agents
  • Ophthalmic Solutions
  • Epidermal Growth Factor