Abstract
Systematic mappings of protein interactome networks have provided invaluable functional information for numerous model organisms. Here we develop PCR-mediated Linkage of barcoded Adapters To nucleic acid Elements for sequencing (PLATE-seq) that serves as a general tool to rapidly sequence thousands of DNA elements. We validate its utility by generating the ORFeome for Oryza sativa covering 2,300 genes and constructing a high-quality protein-protein interactome map consisting of 322 interactions between 289 proteins, expanding the known interactions in rice by roughly 50%. Our work paves the way for high-throughput profiling of protein-protein interactions in a wide range of organisms.
Keywords:
ORFeome; networks; next-generation sequencing; protein–protein interaction; rice.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Computational Biology / methods
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DNA, Plant / genetics
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Databases, Genetic
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Genome, Plant / genetics
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High-Throughput Nucleotide Sequencing / methods
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Open Reading Frames / genetics*
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Oryza / genetics*
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Protein Interaction Mapping / methods*
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Protein Interaction Maps / genetics*
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Sequence Analysis, DNA / methods*