Predictive molecular pathology in the time of COVID-19

J Clin Pathol. 2021 Apr;74(4):234-237. doi: 10.1136/jclinpath-2020-206711. Epub 2020 May 19.

Abstract

Aims: In the time of COVID-19, predictive molecular pathology laboratories must still timely select oncological patients for targeted treatments. However, the need to respect social distancing measures may delay results generated by laboratory-developed tests based on sequential steps a long hands-on time. Laboratory workflows should now be simplified.

Methods: The organisation of the University of Naples Federico II predictive pathology laboratory was assessed before (March-April 2019) and during (March-April 2020) the Italian lockdown.

Results: The number of patients undergoing single or multiple biomarker testing was similar in 2019 (n=43) and in 2020 (n=45). Considering adequate samples for molecular testing, before the outbreak, next-generation sequencing was mostly used (35/42, 83.3%). Testing six genes had a reagent cost of €98/patient. Conversely, in 2020, almost all cases (38/41, 92.7%) were analysed by automated testing. This latter had for any single assay/gene a significant reagent cost (€95-€136) and a faster mean turnaround time (5.3 vs 7.9 working days).

Conclusion: In the times of coronavirus, laboratory fully automated platforms simplify predictive molecular testing. Laboratory staff may be more safely and cost-effectively managed.

Keywords: laboratory personnel; medical oncology; pathology, molecular.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Automation, Laboratory
  • Biomarkers / analysis*
  • COVID-19 / diagnosis*
  • COVID-19 / epidemiology
  • COVID-19 / virology
  • Disease Outbreaks*
  • Female
  • Humans
  • Italy / epidemiology
  • Laboratories
  • Male
  • Medical Oncology*
  • Middle Aged
  • Molecular Diagnostic Techniques
  • Pathology, Molecular*
  • Predictive Value of Tests
  • SARS-CoV-2 / isolation & purification*
  • Time Factors
  • Workflow
  • Young Adult

Substances

  • Biomarkers