Myeloid-derived suppressor cells exert immunosuppressive function on the T helper 2 in mice infected with Echinococcus granulosus

Xiaojing Zhou; Wen Wang; Fang Cui; Chunli Shi; Xiaoping Gao; Jiaxin Ouyang; Yuxin Wang; Atsushi Nagai; Wei Zhao; Mei Yin; Jiaqing Zhao

doi:10.1016/j.exppara.2020.107917

Myeloid-derived suppressor cells exert immunosuppressive function on the T helper 2 in mice infected with Echinococcus granulosus

Exp Parasitol. 2020 Aug:215:107917. doi: 10.1016/j.exppara.2020.107917. Epub 2020 May 22.

Authors

Xiaojing Zhou¹, Wen Wang², Fang Cui², Chunli Shi², Xiaoping Gao³, Jiaxin Ouyang², Yuxin Wang⁴, Atsushi Nagai⁵, Wei Zhao⁶, Mei Yin⁷, Jiaqing Zhao⁸

Affiliations

¹ College of Clinical Medicine, Ningxia Medical University, Yinchuan, 750004, China; Department of Neurology, Shimane University Faculty of Medicine, Izumo, 6938501, Japan.
² School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, 750004, China.
³ Department of Otorhinolaryngology-Head and Neck Surgery, General Hospital of Ningxia Medical University, Yinchuan, 750004, China.
⁴ College of Clinical Medicine, Ningxia Medical University, Yinchuan, 750004, China.
⁵ Department of Neurology, Shimane University Faculty of Medicine, Izumo, 6938501, Japan.
⁶ School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, 750004, China; Center of Scientific Technology, Ningxia Medical University, Yinchuan, 750004, China.
⁷ Cardiovascular Hospital Cardiovascular Hospital, General Hospital of Ningxia Medical University, Yinchuan, 750004, China. Electronic address: [email protected].
⁸ School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, 750004, China; Center of Scientific Technology, Ningxia Medical University, Yinchuan, 750004, China. Electronic address: [email protected].

PMID: 32446699
DOI: 10.1016/j.exppara.2020.107917

Abstract

Cystic echinococcosis (CE) is a worldwide hazardous zoonotic parasitosis caused by Echinococcus granulosus. CE development involves complex immunological mechanisms, including participation of multiple immune cells and effector molecules. Myeloid-derived suppressor cells (MDSCs) are known to be involved in chronic and acute inflammatory conditions. In this study, we aimed to characterize the immune function of MDSCs in CE to improve the understanding, prevention and treatment of CE. Our results indicated that MDSCs overexpressing Ly6C and Ly6G inhibit the formation and activity of T helper 2 cells in a NO-dependent manner during E. granulosus infection.

Keywords: Echinococcus granulosus; Immunosuppressive function; Myeloid-derived suppressor cells (MDSCs).

MeSH terms

Analysis of Variance
Animals
Antibodies, Monoclonal
Arginase / analysis
Bone Marrow / drug effects
Bone Marrow / immunology
Cytokines / analysis
Echinococcosis / immunology*
Echinococcus granulosus / immunology*
Female
Flow Cytometry
Humans
Keratolytic Agents / pharmacology
Mice
Myeloid-Derived Suppressor Cells / drug effects
Myeloid-Derived Suppressor Cells / enzymology
Myeloid-Derived Suppressor Cells / immunology*
Nitric Oxide / analysis
Reactive Oxygen Species / analysis
Specific Pathogen-Free Organisms
Spleen / cytology
Spleen / drug effects
Spleen / immunology
Th2 Cells / immunology*
Tretinoin / pharmacology

Substances

Antibodies, Monoclonal
Cytokines
Keratolytic Agents
Reactive Oxygen Species
Nitric Oxide
Tretinoin
Arginase