Alleviation of drought stress and the physiological mechanisms in Citrus cultivar (Huangguogan) treated with methyl jasmonate

Biosci Biotechnol Biochem. 2020 Sep;84(9):1958-1965. doi: 10.1080/09168451.2020.1771676. Epub 2020 May 25.

Abstract

The role of exogenous methyl jasmonate (MeJA) in alleviating drought stress was investigated on Huangguogan. Except for intercellular CO2 concentration, MeJA had little effect on net photosynthetic rate, stomatal conductance, and transpiration rate under drought stress. Compared with drought stress, MeJA significantly alleviated the decrease of chlorophyll content. However, chlorophyll a/b ratio was significantly increased. MeJA significantly increased proline and soluble sugar contents, significantly decreased the O2 and H2O2 levels, and increased SOD and POD activities. In addition, the MDA content of drought stress was the highest of all treatments. MeJA significantly reduced MDA content in drought-stressed Huangguogan leaves. Although the Ascorbic acid (AsA) contents of 500 and 1000 mg L-1 MeJA treatments were lower than that of 250 mg L-1 MeJA, but all concentration of MeJA treatments delayed the decline of AsA content. Therefore, MeJA could induce drought stress tolerance by increasing the osmotic adjustment substances and antioxidant activities.

Keywords: Citrus; antioxidant activity; drought stress; methyl Jasmonate; photosynthesis.

MeSH terms

  • Acetates / pharmacology*
  • Ascorbic Acid / metabolism
  • Carbon Dioxide / metabolism
  • Citrus / cytology
  • Citrus / drug effects*
  • Citrus / physiology*
  • Cyclopentanes / pharmacology*
  • Droughts*
  • Hydrogen Peroxide / metabolism
  • Intracellular Space / drug effects
  • Intracellular Space / metabolism
  • Malondialdehyde / metabolism
  • Oxylipins / pharmacology*
  • Signal Transduction / drug effects
  • Stress, Physiological / drug effects*

Substances

  • Acetates
  • Cyclopentanes
  • Oxylipins
  • Carbon Dioxide
  • Malondialdehyde
  • methyl jasmonate
  • Hydrogen Peroxide
  • Ascorbic Acid