Using the Patterned Microarray Culture to Obtain Gene-Editing Monoclonal Cells

Yunlin Zhang; Mengyu Gao; Meijuan Hu; Bingqi Zhang; Yuting He; Shun Li; Ji Bao; Hong Bu

doi:10.1016/j.transproceed.2020.02.138

Using the Patterned Microarray Culture to Obtain Gene-Editing Monoclonal Cells

Transplant Proc. 2020 Jul-Aug;52(6):1906-1909. doi: 10.1016/j.transproceed.2020.02.138. Epub 2020 May 24.

Authors

Yunlin Zhang¹, Mengyu Gao², Meijuan Hu², Bingqi Zhang¹, Yuting He¹, Shun Li³, Ji Bao⁴, Hong Bu²

Affiliations

¹ Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital, Sichuan University, Chengdu, China.
² Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital, Sichuan University, Chengdu, China; Department of Pathology, West China Hospital, Sichuan University, Chengdu, China.
³ Department of Biophysics, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
⁴ Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital, Sichuan University, Chengdu, China. Electronic address: [email protected].

PMID: 32461005
DOI: 10.1016/j.transproceed.2020.02.138

Abstract

Genetically modified pigs are the first choice for xenotransplantation research, but there have been problems with monoclonal screening of edited cells before nuclear transfer. Our objective was to get a novel strategy to quickly obtain monoclonal cells with low damage by microarray and to produce efficient gene-editing monoclonal cells in batches. Micropattern array printing technology was introduced to limit only a single cell was adhered on a micropattern substrate, and after 4 days of culture, the single cell grew into a monoclonal cell sphere and then came off from the bottom of the petri dish automatically. After sequencing, the results showed that a single cell is confined to a micropattern and grows into a sphere of monoclonal cells.

MeSH terms

Animals
Cell Culture Techniques / methods*
Clone Cells
Gene Editing / methods*
Microarray Analysis / methods*
Swine