Abnormalities of aquaporin-4 in the cerebellum in bipolar II disorder: An ultra-high b-values diffusion weighted imaging study

Background: Cumulative evidence indicated the cerebellum is involved in the pathophysiology of bipolar disorder (BD). It was reported that the apparent diffusion coefficient from ultra-high b-values (ADC_uh) could reflect the function of aquaporin-4 (AQP4) which was involved in neurological disorders. However, no studies have reported the AQP4 alteration in the cerebellum in BD. Therefore, this study aimed to investigate the ADC_uh and AQP4 in the cerebellum in BD-II.

Methods: Fifty patients with BD-II as well as 43 healthy controls underwent enhance diffusion weighted imaging (eDWI) with ultra-high b-values. The eDWI parameters including ADC_uh , pure water diffusion (D) and pseudodiffusion (D*) was measured using regions-of-interest analysis in the superior cerebellar peduncles (SCP), middle cerebellar peduncles (MCP) , cerebellar hemisphere, dentate nuclei, tonsil and vermis of the cerebellum.

Results: BD-II exhibited increased ADC_uh values in the bilateral SCP, cerebellar hemisphere, tonsil and right dentate nuclei, and increased D* and D in the bilateral SCP, and decreased D* in the tonsil. Additionally, there were positive correlations between Hamilton Rating Scale for Depression-24 scores and bilateral ADC_uh values in the SCP and cerebellar hemisphere.

Conclusions: The alteration of the ADC_uh values in the cerebellum may reflect the changes of the AQP4, especially the abnormality of eDWI parameters in the SCP may be a key neurobiological feature of BD-II. The current results provide a novel insight to look into the pathophysiology mechanisms underlying BD-II.