Interactive effects of elevated CO₂ concentration and combined heat and drought stress on tomato photosynthesis

Background: Extreme weather events are predicted to increase, such as combined heat and drought. The CO₂ concentration ([CO₂]) is predicted to approximately double by 2100. We aim to explore how tomato physiology, especially photosynthesis, is affected by combined heat and drought under elevated [CO₂] (e [CO₂]).

Results: Two genotypes, 'OuBei' ('OB', Solanum lycopersicum) and 'LA2093' (S. pimpinellifolium) were grown at a [CO₂] (atmospheric [CO₂], 400 ppm) and e [CO₂] (800 ppm), respectively. The 27-days-old seedlings were treated at 1) a [CO₂], 2) a [CO₂] + combined stress, 3) e [CO₂] and 4) e [CO₂] + combined stress, followed by recovery. The P_N (net photosynthetic rate) increased at e [CO₂] as compared with a [CO₂] and combined stress inhibited the P_N. Combined stress decreased the F_v/F_m (maximum quantum efficiency of photosystem II) of 'OB' at e [CO₂] and that of 'LA2093' in regardless of [CO₂]. Genotypic difference was observed in the e [CO₂] effect on the gas exchange, carbohydrate accumulation, pigment content and dry matter accumulation.

Conclusions: Short-term combined stress caused reversible damage on tomato while the e [CO₂] alleviated the damage on photosynthesis. However, the e [CO₂] cannot be always assumed have positive effects on plant growth during stress due to increased water consumption. This study provided insights into the physiological effects of e [CO₂] on tomato growth under combined stress and contributed to tomato breeding and management under climate change.