Serodiagnosis of kala-azar was determined by dot-ELISA under variable conditions such as may be encountered in the field, for example, in the nature of the antigen, the preservation of antigen at different temperatures, variation in incubation temperature and so on. The test was found to be useful under field conditions, and the correlation between ELISA and dot-ELISA was significant. Dot-ELISA has definite advantages over the ELISA test, as it is easy to perform, is stable and does not require expensive equipment. The test can be used for large-scale screening of sera in sero-epidemiological studies.