[Cyanate-induced oxidative stress injury in human normal liver HL-7702 cells]

L Hu; K Tian; T Zhang; C H Fan; P Zhou; D Z Yuan; Y Chen; J Li; J H Ran

doi:10.3760/cma.j.cn501113-20190801-00283

[Cyanate-induced oxidative stress injury in human normal liver HL-7702 cells]

Zhonghua Gan Zang Bing Za Zhi. 2020 May 20;28(5):446-449. doi: 10.3760/cma.j.cn501113-20190801-00283.

[Article in Chinese]

Authors

L Hu¹, K Tian¹, T Zhang¹, C H Fan¹, P Zhou², D Z Yuan³, Y Chen², J Li², J H Ran⁴

Affiliations

¹ Neuroscience Research Platform of Basic Medical College of Chongqing Medical University, Chongqing 400016, China.
² Laboratory of Histology and Cell Engineering and Stem Cells, Basic Medical College of Chongqing Medical University, Chongqing 400016, China.
³ Department of Neurology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
⁴ Neuroscience Research Platform of Basic Medical College of Chongqing Medical University, Chongqing 400016, China; Laboratory of Histology and Cell Engineering and Stem Cells, Basic Medical College of Chongqing Medical University, Chongqing 400016, China.

PMID: 32536063
DOI: 10.3760/cma.j.cn501113-20190801-00283

Abstract

研究采用氰酸盐负荷人正常肝细胞HL-7702，结果显示，氰酸盐可引起细胞内活性氧（ROS）含量升高；细胞内的抗氧化酶如超氧化物歧化酶和过氧化氢酶在氰酸盐处理后显著下降；细胞产生的脂质过氧化反应的终产物丙二醛和高反应性自由基一氧化氮在经氰酸盐负荷后显著升高；此外，细胞内核因子E2相关因子2、血红素加氧酶1及一氧化氮合成酶蛋白水平下调、肝细胞内脂滴明显增加。由此可见，氰酸盐可以打破肝细胞内氧化应激平衡，ROS大量产生，肝细胞内脂质沉积。.

Keywords: Cyanate; Hepatocytes; Lipid; Reactive oxygen.

MeSH terms

Cyanates / metabolism
Cyanates / toxicity
Hepatocytes
Humans
Liver* / metabolism
Oxidative Stress*
Reactive Oxygen Species / metabolism

Substances

Cyanates
Reactive Oxygen Species