Citrus reticulata Blanco peel extract ameliorates hepatic steatosis, oxidative stress and inflammation in HF and MCD diet-induced NASH C57BL/6 J mice

Zunli Ke; Yuanyuan Zhao; Si Tan; Hui Chen; Yin Li; Zhiqin Zhou; Cheng Huang

doi:10.1016/j.jnutbio.2020.108426

Citrus reticulata Blanco peel extract ameliorates hepatic steatosis, oxidative stress and inflammation in HF and MCD diet-induced NASH C57BL/6 J mice

J Nutr Biochem. 2020 Sep:83:108426. doi: 10.1016/j.jnutbio.2020.108426. Epub 2020 May 22.

Authors

Zunli Ke¹, Yuanyuan Zhao², Si Tan³, Hui Chen², Yin Li², Zhiqin Zhou⁴, Cheng Huang⁵

Affiliations

¹ Morphological Laboratory, Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou 550025, China; Drug Discovery Lab, School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China.
² Drug Discovery Lab, School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
³ School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Fuling, 408100, Chongqing, China.
⁴ College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China.
⁵ Drug Discovery Lab, School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. Electronic address: [email protected].

PMID: 32559586
DOI: 10.1016/j.jnutbio.2020.108426

Abstract

Excessive lipid deposition, oxidative stress and inflammation in liver tissues are regarded as crucial inducers of nonalcoholic steatohepatitis (NASH), which is the most frequent chronic liver disease and closely related to obesity and insulin resistance. In this work, the preventive and therapeutic effects of Citrus reticulata Blanco (Jizigan) peel extract (JZE) on NASH induced by high fat (HF) diet and methionine choline-deficient (MCD) diet in C57BL/6 mice were investigated. We found that daily supplementation of JZE with an HF diet effectively ameliorated glucose tolerance and insulin resistance. In addition, the key indexes of lipid profiles, oxidative stress, hepatic steatosis and inflammatory factors were also ameliorated in both NASH mouse models. Furthermore, JZE treatment activated nuclear factor erythroid-2-related factor 2 (Nrf2) in the livers of diet- induced NASH mice. Our study suggests that JZE might alleviate NASH via the activation of Nrf2 signaling and that citrus Jizigan could be used as a dietary therapy for NASH and related metabolic syndrome.

Keywords: Citrus reticulata Blanco peel extract; Inflammation; Lipid deposition; NASH; Nrf2; Oxidative stress.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Choline / analysis*
Choline / metabolism
Citrus / chemistry*
Diet, High-Fat / adverse effects
Disease Models, Animal
Fruit / chemistry
Humans
Liver / drug effects
Liver / immunology
Male
Methionine / analysis*
Methionine / deficiency
Mice
Mice, Inbred C57BL
NF-E2 Transcription Factor / genetics
NF-E2 Transcription Factor / immunology
Non-alcoholic Fatty Liver Disease / drug therapy*
Non-alcoholic Fatty Liver Disease / genetics
Non-alcoholic Fatty Liver Disease / immunology*
Oxidative Stress / drug effects*
Plant Extracts / administration & dosage*

Substances

NF-E2 Transcription Factor
Plant Extracts
Methionine
Choline