Chitosan-based macroparticle is a common carrier for enzyme immobilization applied in food industry. Driven by the requirement of large carrier pores for the biomacromolecular substrates such as protein, the eggshell membrane powder (ESMP) was employed as multifunctional porogen to improve the physicochemical structure of chitosan-based macroparticles. The prepared macroparticles were characterized by SEM, XRD, FTIR, Raman spectroscopy, nitrogen adsorption-desorption isotherms, and thermogravimetric analysis. The results showed that an increase of ESMP percentage could improve the porosity of macro holes in macroparticles, and it also enlarged the size of mesopores. Moreover, the ESMP significantly increased (P < 0.05) the amount of papain immobilization, whereas the specific activity of immobilized papain achieved a maximum value of 871.95 U/mg at CSESM2 and then declined with the increase of ESMP. Therefore, the inclusion of 20 % ESMP in chitosan-based macroparticles gave the highest activity of its immobilized protease.
Keywords: Chitosan-based macroparticles; Eggshell membrane; Macroporous structure; Protease immobilization; Substrate diffusion.
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