A novel rapid method based on a background fluorescence quenching immunochromatographic assay (bFQICA) was established to achieve simultaneously the quantitative detection of 3-methyl-quinoxaline-2-carboxylic acid (MQCA) and quinoxaline-2-carboxylic acid (QCA), which were efficiently extracted and enriched 4 times using immunomagnetic beads from pork. The analysis of field pork samples by bFQICA was in accordance with that of LC-MS/MS; especially, the proposed bFQICA exhibited great advantages in convenience and efficiency, which only takes 30 min for the detection of MQCA and QCA.
Keywords: 3-methyl-quinoxaline-2-carboxylic acid (MQCA); Background fluorescence quenching immunochromatographic assay (bFQICA); immunomagnetic beads; pork; quinoxaline-2-carboxylic acid (QCA).